MBT-24H Newly Released Product !! RBT-24H RBE-12 (PET-12) How to Use MBT-24H Newly Released Product !! How to Use RBT-24H How to Use RBE-12 Drug Permeability Assay

HOME>Products>RBT-24H BBB Kit™ ( RBT-24H ) / ( RBT-24F )* For Drug BBB Permeability Study ( from micromolecules to cells ) BBB Kit™ (RBT-24) is a new in vitro model of the blood-brain barrier (BBB) made of primary cultures of rat (Wistar rat) brain capillary endothelial cells, brain pericytes and astrocytes. BBB Kit™ (RBT-24) with a polyethylene terephthalate (PT) membrane is a ready-to-use concept for your evaluations of BBB-permeability of drugs and drug candidates.  BBB Kit™ (RBT-24H) is a 12-well format constructed in a 24-well Millicell® cell culture plate. ( *On-Demand Product - RBT-24F ) BBB Kit™ (RBT-24H) is constructed in 12 wells (B and C) with Millicell® Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell® plate. [ H = Half ] *For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit™ in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our calculation of apparent permeability is Papp. **Cells within BBB Kit™ (RBT-24) are not clearly seen by microscopic observations. ***PT membrane hardly absorbs chemicals. We deliver the complete set of BBB Kit™ (RBT-24H) in frozen packaged with dry ice. BBB Kit™ can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit (PRBT-24H) within 1 month. BBB Kit ™ (RBT-24H) Contents: BBB Kit ™ (RBT-24H) Thawing - and Incubation - Medium Millicell® Hanging Cell Culture Insert 1 (for Blank experiments)   Catalog # Product Format Unit Price（Yen） PCC-RBT-24H BBB Kit ™ (RBT-24H) 12 inserts Set 148,000 Ready-To-Use BBB Kit ™ We deliver the complete set of BBB Kit ™ in frozen packaged with dry ice. BBB Kit ™ can be frozen as a whole and stored at -80℃. We guarantee to use BBB Kit ™ (RBT-24H) within 1 month. 4 days prior to your experiment, you just thaw (de-freeze) your BBB Kit ™ (RBT-24H) stored at -80℃ in your deep-freezer.     Amounts of solutions (to be added)   Thawing-sol medium (1) medium (2)  * Blood-side 200 μL 300 μL 300 μL Brain-side 1000 μL 1200 μL 1200 μL * Recipe: medium 10% PDS / DMEM F-12   Dulbecco's Modified Eagle's Medium/ Nutrient Mixture F-12 Ham (DMEM F-12)   Fetal Bovine Plasma Derived Serum (PDS) 10% (v/v) Heparin 100 μg/mL basic fibroblast growth factor (bFGF) 1.5 ng/mL Insulin-Transferrin-sodium Selenite (ITS) insulin 5 μg/mL, transferrin 5 μg/mL, sodium selenite 5 ng/mL Hydrocortisone 500 nM Gentamicin 50 μg/mL Trans Endothelial Electrical Resistance (TEER) of BBB Kit ™ (RBT-24) TEER in BBB Kit™ (RBT-24) reaches more than 150 Ω x cm2, and maintains a plateau up to 6 days. (Medium does not contain cAMP and its analogs.) TEER is measured at Day 3 after changing medium. BBB Kit™ (RBT-24) can be used from Day 4 to Day 6 after thawing.   【Equipment】 Insert Membrane Infomation Insert of BBB Kit™ (RBT-24H) (Polyethylene terephthalate membrance filter) Millicell® Hanging Cell Culture Insert (#PISP 12R 48) Technical data of the polyethylene terephthalate (PT )membrane Millicell® Insert (#PISP 12R 48) Diameter: 6.5 mm Growth area: 0.33 cm2 Membrane thickness: 9 μm Pore size: 3.0 μm Pore density: 2 x 106 pores / cm2 Tissue culture treated: yes Optical property: translucent Cell visibility: poor Volume inside insert: 0.2 mL - 0.3 mL Volume in plate: 0.9 mL - 1.2 mL Millicell® is a Registered Trade Mark of Millipore Corporation. BBB Kit™ ( RBT-24F ) BBB Kit™ (RBT-24F) is constructed in 24 wells with Millicell® Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell® plate. [ F = Full ] *For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit™ in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our Calculation of apparent permeability is Papp. **Cells within BBB Kit™ (RBT-24) are not clearly seen by microscopic observations. ***PT membrane hardly absorbs chemicals. We deliver the complete set of BBB Kit™ (RBT-24F) in frozen packaged with dry ice. BBB Kit™ can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit (RBT-24F) within 1 month. BBB Kit ™ (RBT-24F) Contents: BBB Kit ™ (RBT-24F) Thawing - and Incubation - medium   Catalog # Product Format Unit Price (Yen) PCC-RBT-24F (On-Demand Product) BBB Kit ™ (RBT-24F) 24 inserts Set 198,000 ⇑ Back to this top