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| BBB Kit™ ( RBE-12 ) (PET-12) |
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| For BBB Cells Analysis / Disease Model / Drug BBB Permeability Study |
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| BBB Kit™ ( RBE-12 ) is a new in vitro model of the blood-brain barrier (BBB) made of primary cultures of rat (Wistar rat) brain capillary endothelial cells, brain pericytes and astrocytes. BBB Kit™( RBE-12 ) with polyester (PE) membrane** is a ready-to-use concept for your researches on BBB-related physiology and evaluations of BBB permeability of your drugs and drug candidates. |
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*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit™ in blood-to-brain direction, the molecule is applied to the upper compartment of the insert.
Our calculation of apparent permeability is Papp or Pe. However, depending on the compound, you may not be able to caltulate Papp or Pe exactly, because of absorption of the compound to polyester membrane. In such a case, you can try our other types of our BBB Kit™ ( MBT-24 / RBT-24 ).
**Endothelial cells on polyester membrane can be clearly observed by an inverted microscope.
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We deliver the complete set of BBB Kit™ (RBE-12) in frozen packaged with dry ice.
BBB Kit™ can be frozon as a whole and stored at -80 ℃. We gurantee to use BBB Kit™ (PBE-12) within 1 month. |
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Contents:
- BBB Kit ™ (RBE-12)
- Thawing-solution
- Meduim 1
- Medium 2
- Transwell® (#3460) for Control
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| Catalog # |
Product |
Format |
Unit |
Price（Yen） |
| PCC-RBE-12 |
BBB Kit ™
(RBE-12) |
12 well |
Set |
198,000 |
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4 days prior to your experiment, you just thaw (de-freeze) your BBB Kit ™ (RBE-12) stored at -80℃.
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Amounts of solutions (to be added)
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Thawing-sol |
medium 1 |
medium 2 * |
| Blood-side |
630 μL |
500 μL |
500 μL |
| Brain-side |
1000 μL |
1500 μL |
1500 μL |
* Recipe: medium 2 10% PDS / DMEM F-12
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Dulbecco’s Modified Eagle’s Medium/ Nutrient Mixture F-12 Ham (DMEM F-12) |
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| Fetal Bovine Plasma Derived Serum (PDS) |
10% (v/v) |
| Heparin |
100 μg/mL |
| basic fibroblast growth factor (bFGF) |
1.5 ng/mL |
| Insulin-Transferrin-sodium Selenite (ITS) |
insulin 5 μg/mL,
transferrin 5 μg/mL,
sodium selenite 5 ng/mL |
| Hydrocortisone |
500 nM |
| Gentamicin |
50 μg/mL |
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| Trans Endothelial Electrical Resistance (TEER) of BBB Kit ™ (RBE-12) |
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| TEER in BBB Kit™ (RBE-12) reaches more than 150 Ω x cm2, and maintains a plateau up to 7 days. (Thawing-sol and medium 1, 2 do not contain cAMP and its analogs.) TEER is measured at Day 3 after changing solution and medium. BBB Kit™ (RBE-12) can be used from Day 4 to Day 6 after thawing. |
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【Equipment】
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| Insert Membrane Infomation |
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Insert of BBB KitTM (RBE-12) （Polyester membrane filter）
| Transwell® Permeable Support (#3460) |
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| Technical data of the polyester (PE) membrane Transwell® clear (Corning) insert: |
| Diameter: |
12 mm |
| Growth area: |
1.12 cm2 |
| Membrane thickness: |
10 μm |
| Pore size: |
0.4 μm |
| Pore density: |
4 x 106 pores/cm2 |
| Tissue culture treated: |
yes |
| Coating: |
collagen type IV and fibronectin |
| Optical property: |
clear |
| Cell visibility: |
good |
| Volume inside insert: |
0.5 mL |
| Volume in plate: |
1.5 mL |
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| Transwell® is a Registered Trade Mark of Corning Incorporated. |
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MBT-24H
