MBT-24H Newly Released Product !! RBT-24H RBE-12 (PET-12) How to Use MBT-24H Newly Released Product !! How to Use RBT-24H How to Use RBE-12 Drug Permeability Assay

HOME>Products>Drug Permeability Assay Drug Permeability Assay Drug BBB-Permeability Assay [MBT-24/RBT-24 Drug Permeability Assay Protocol] [ PDF-0.97MB ] For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit ™ in blood-to-brain direction, the molecule is aplied to the upper compartment of the insert. 　　　*Depending on types of molecules or sensitivity measurement, the time points are decided. Schematic Diagram １ *The value obtained with only Transwell® membrane is the background data. The backgound data is needed in the calculation of apparent permeability. Schematic Diagram ２（ex.） Collecting samples t=0 ｔ=20 t=40 t=60 luminal ○ （assay buffer） - - ○ abluminal ○ （assay buffer） ○ ○ ○ cell + membrane − - - ○ （if needs） Analysis １．Calculation of apparant permeability   ２．Evaluation of Papp We obtained a very good correlation between the BBB Kit™ and in vivo permeabilities of drugs. You can evaluate the BBB-permeability by our BBB Kit™, quantitatively. When you design molecular modifications of your compound or vectors carrying your compound into the brain, you can easily evaluate the BBB-permeability, quantitatively.   Papp（×10-6cm/s） Permeability ex) Permeabilitis >20 very good Antipyrine 10〜20 good - 2〜10 low Na-F <2 very low ESA, sucrose *Transwell® is trademark of Corning, Incorporated, Corning, NY, USA. ⇑ Back to this top